ChromoTek EGFP, recombinant purified protein

Purified recombinant Enhanced Green Fluorescent Protein (EGFP); full length protein; fused to 6xHis-tag.

Protein

EGFP

Applications

IP, WB, ELISA

Conjugate

Unconjugated

Cat no : EGFP

Synonyms

EGFP



Product Information

Purified recombinant Enhanced Green Fluorescent Protein (EGFP); full length protein; fused to 6xHis-tag.

ApplicationsImmunoprecipitation: use ~ 5 μg EGFP per 25 μL GFP-Trap® bead slurry
SDS-PAGE: use ~ 1 μg EGFP per lane
Western blot: use ~ 25 ng - 100 ng EGFP per lane
ELISA: coat ~ 1ng - 50 ng per well (96-well plate)
Other in vitro assays: as a fluorescent standard and for calibration
Molecular Weight28.0 kDa (including the polyhistidine-tag)
FormLiquid
Size250 µL
Concentration1 g/L
ConjugateUnconjugated
Purity> 80% by SDS-PAGE
SourceEscherichia coli
Storage Buffer0.09% sodium azide
Storage ConditionShipped at +4°C.
Store at +4°C short term (up to 1 week).
Store at -20°C or -80°C long term.
Avoid freeze-thaw cycles. Stable for one year.

Publications

ApplicationTitle

Cell

Cellular Control of Viscosity Counters Changes in Temperature and Energy Availability.

Authors - Laura B Persson
IP

PLoS Pathog

New insights into the role of endosomal proteins for African swine fever virus infection.

Authors - Miguel Ángel Cuesta-Geijo