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RFP-Trap

Description RFP-Trap a versatile tool for biochemical and cell biological analysis of RFP-tagged fusion proteins.
Specificity RFP-Trap efficiently pulls down various Red fluorescent proteins derived from DsRed, e.g. mRFP1, mCherry, mOrange, mPlum but also mRuby and RFP-tagged fusion proteins.

Tested Applications

Pulldown:

Protocol (PDF)

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Form and Storage Buffer RFP-Trap_A RFP-Trap coupled to agarose beads
particle size ~ 80 µm
stored in 20% EtOH
RFP-Trap_M RFP-Trap coupled to magnetic particles
particle size 0.5 - 1 µm (particles from chemicell)
stored in 1x PBS, 0.1% BSA
RFP-Trap uncoupled,
purified protein
modified on demand (e.g. biotinylated)
Size
RFP-Trap_A 20 reactions (500 µl resin) rta-20
100 reactions (2.5 ml resin) rta-100
200 reactions (5 ml resin) rta-200
400 reactions (10 ml resin) rta-400
RFP-Trap_A Kit 20 reactions (500 µl resin) rtak-20
RFP-Trap_M 20 reactions (500 µl resin) rtm-20
100 reactions (2.5 ml resin) rtm-100
200 reactions (5 ml resin) rtm-200
400 reactions (10 ml resin) rtm-400
RFP-Trap_M Kit 20 reactions (500 µl resin) rtmk-20
RFP-Trap 250 µl rt-250
Storage instructions Shipped at RT. Store at 4°C. Do not freeze.
 

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Only for research applications, not for diagnostic or therapeutic use.