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RFP-Trap®

Description RFP-Trap® a versatile tool for biochemical and cell biological analysis of RFP-tagged fusion proteins.
Specificity RFP-Trap® efficiently pulls down various Red fluorescent proteins derived from DsRed, e.g. mRFP1, mCherry, mOrange, mPlum but also mRuby and RFP-tagged fusion proteins.

Tested Applications

Pulldown:

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RFP-Trap®_A
RFP-Trap® coupled to
agarose beads
particle size ~ 80 µm
stored in 20% EtOH

Protocol (PDF)		 20 reactions (500 µl resin) rta-20
100 reactions (2.5 ml resin) rta-100
200 reactions (5 ml resin) rta-200
400 reactions (10 ml resin) rta-400
RFP-Trap®_A Kit
including lysis, wash
and elution buffers



Protocol (PDF)		 20 reactions (500 µl resin) rtak-20
   
RFP-Trap®_M
RFP-Trap® coupled to
magnetic particles
particle size 0.5 - 1 µm (particles from chemicell)
stored in 1x PBS, 0.1% BSA
Protocol (PDF)		 20 reactions (500 µl resin) rtm-20
100 reactions (2.5 ml resin) rtm-100
200 reactions (5 ml resin) rtm-200
400 reactions (10 ml resin) rtm-400
RFP-Trap®_M Kit
including lysis, wash
and elution buffers



Protocol (PDF)		 20 reactions (500 µl resin) rtmk-20
   
RFP-Trap®
uncoupled,
purified protein
modified on demand
(e.g. biotinylated)

 250 µl rt-250
   
Product brochure (PDF)
Storage instructions Shipped at RT. Store at 4°C. Do not freeze.

recommended complements/ related products:

  • 3H9 - Antibody to Green Fluorescent Protein GFP (rat monoclonal) *** NEW ***
  • 5F8 - Antibody to Multiple Red Fluorescent Proteins (rat monoclonal)
  • 16D10 - Antibody to Proliferation Marker PCNA (rat monoclonal)

Only for research applications, not for diagnostic or therapeutic use.