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GFP-Booster_Atto488

  code price size
  gba488 €250.00 100 µg
Description GFP-Trap® coupled to fluorescent dye ATTO 488

Relevance

GFP is a powerful tool to study protein localization and dynamics in living cells. However, the photo stability and the quantum efficiency of GFP is not sufficient for Super Resolution Microscopy (e.g. 3D-SIM or STED) of fixed samples from cells expressing GFP-fusion proteins to visualize specific structures. Furthermore, many cell biological methods such as HCl treatment for BrdU-detection, the EdU-Click-iT™ treatment or heat denaturation for FISH lead to disruption of GFP signal.
Use GFP-Booster_Atto488, a specific GFP-binding protein coupled to the fluorescent dye ATTO 488 (from ATTO-TEC) for reactivation, boosting and stabilization of GFP.

Specificity

GFP-Trap® efficiently detects and labels green fluorescent protein from Aequorea victoria and derivates thereof such as eGFP or eYFP but not eCFP.
Tested Applications and Recommended Dilutions
Immunofluorescence
 
Enhancement of GFP signal with GFP-Booster after EdU-Click-iT™ treatment. EdU-Click-iT™ treament leads to disruption of GFP signal. GFP-Booster labels GFP fusion proteins and thus reactivates and boosts the fluorescence.  
 
 
Enhancement of GFP signal with GFP-Booster_Atto488.
Comparison of signal intensity of a HeLa cell line stably expressing a nuclear GFP-fusion protein before and after GFP-Booster treatment.		  

Optimal dilutions/concentrations should be determined by the end user.
Reference Guizetti, J., Schermelleh, L., et al. (2011). Cortical Constriction During Abscission Involves Helices of ESCRT-III-Dependent Filaments. Science. (PubMed)
Concentration 1 mg/ml
Storage instructions Shipped at RT. Store at 4°C, do not freeze. Protect from light.
 

Only for research applications, not for diagnostic or therapeutic use.