products GFP-Trap® RFP-Trap® Booster Chromobodies®   ***new*** Monoclonal antibodies Binding controls

Products > Monoclonal antibodies > GFP antibody

  code price size
  3h9 €170.00 100 µg
Description Rat monoclonal [3H9] to Green Fluorescent Protein.
For biochemical and cell biological analysis of GFP-tagged fusion proteins.
Product Type Primary antibody
Specificity 3H9 recognizes green fluorescent protein GFP.

Isotype

 IgG2a

Relevance

Fluorescent proteins (FP) are powerful tools to study protein localization and dynamics in living cells In addition to the prominent green fluorescent protein (GFP) from Aequorea victoria.
Here we offer a rat monoclonal antibody (MAb) against green FPs, designated as 3H9. The 3H9 MAb is characterized by high affinity and specificity against GFP. It is suitable for immunoblotting and ELISA.
With this versatile antibody GFP and fusions thereof become attractive tools for biochemical applications like identification of interacting proteins or Western Blotting.

Tested Applications and Recommended Dilutions

Western Blot: Used at a dilution of 1/1000. Detects a band of approximately 31 kDa (the predicted molecular weight of GFP).
primary antibody: 0.2 µg of GFP antibody 3H9
incubation for 1 h at RT
secondary antibody: anti-rat-HRP (Jackson ImmunoResearch Europe)
detection with ECL Western Blot Detection Kit

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Form Liquid; hybridoma supernatant. The hybridoma was cultured in protein free medium. Tissue culture supernatant was concentrated by centrifugation using a 30 kD exclusion membrane to an antibody concentration of 1 mg/ml.
Storage Buffer Preservative: 0.01% Sodium Azide
Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
Concentration 1 mg/ml
Storage instructions Shipped at RT. Upon delivery aliquot and store at -20°C. Store at 4°C short term (1-2 weeks). Avoid freeze / thaw cycles.
 

Only for research applications, not for diagnostic or therapeutic use.