Nano-Boosters & Nano-Labels

ChromoTek's Nano-Boosters & Nano-Labels are fluorescent probes. They consist of very small recombinant antibody domains, also called Nanobodies or VHHs. These single-domain alpaca antibody fragments are covalently coupled to fluorescent dyes.

  • The Nano-Boosters GFP- and RFP-Boosters enhance the signal of fluorescent proteins
  • The Nano-Labels Vimentin- and Histone-Label fluorescently label endogenous cellular proteins
  • The Spot-Label fluorescently labels the Spot-Tag, a 12-aa peptide tag

Applications
Immunofluorescence (IF): immunocytochemistry (ICC) and immunohistochemistry (IHC)
Cleared tissue, organ, and animal (DISCO) staining for light-sheet microscopy imaging
Super-resolution microscopy (SRM)

    GFP-Booster

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    CodeDescriptionSizePriceQty
    Codegba488-10DescriptionGFP-Booster conjugated to ATTO488Size10 µLPrice $ 100
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    Codegba488-100DescriptionGFP-Booster conjugated to ATTO488Size100 µLPrice $ 365
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    Codegba594-10DescriptionGFP-Booster conjugated to ATTO594Size10 µLPrice $ 100
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    Codegba594-100DescriptionGFP-Booster conjugated to ATTO594Size100 µLPrice $ 365
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    Codegba647n-10DescriptionGFP-Booster conjugated to ATTO647NSize10 µLPrice $ 110
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    Codegba647n-100DescriptionGFP-Booster conjugated to ATTO647NSize100 µLPrice $ 395
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    CodegbAF647-10DescriptionGFP-Booster conjugated to Alexa Fluor® 647Size10 µLPrice $ 110
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    CodegbAF647-100DescriptionGFP-Booster conjugated to Alexa Fluor® 647Size100 µLPrice $ 395
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    Codegbas635p-10DescriptionGFP-Booster conjugated to Abberior AS 635PSize10 µLPrice $ 100
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    Codegbas635p-100DescriptionGFP-Booster conjugated to Abberior AS 635PSize100 µLPrice $ 365
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    Codegt-250DescriptionGFP-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
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    CodecustomDescriptionGFP-Binding protein, custom labelingSizePrice Please inquire

    Histone-Label

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    CodeDescriptionSizePriceQty
    Codetba488-10DescriptionHistone-Label conjugated to Atto488Size10 µLPrice $ 100
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    Codetba488-100DescriptionHistone-Label conjugated to Atto488Size100 µLPrice $ 365
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    Codett-250DescriptionHistone-Binding-protein, 1 mg/mLSize250 µLPrice $ 275
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    RFP-Booster

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    CodeDescriptionSizePriceQty
    Coderba594-10DescriptionRFP-Booster conjugated to ATTO594Size10 µLPrice $ 100
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    Coderba594-100DescriptionRFP-Booster conjugated to ATTO594Size100 µLPrice $ 365
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    Coderba647n-10DescriptionRFP-Booster conjugated to ATTO647NSize10 µLPrice $ 110
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    Coderba647n-100DescriptionRFP-Booster conjugated to ATTO647NSize100 µLPrice $ 395
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    Codert-250DescriptionRFP-Binding-Protein, 1 mg / mLSize250 µLPrice $ 275
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    CodecustomDescriptionRFP-Binding protein, custom labelingSizePrice Please inquire

    Spot-Label

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    CodeDescriptionSizePriceQty
    Codeeba488-10DescriptionSpot-Label conjugated to Atto488Size10 µLPrice $ 100
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    Codeeba488-50DescriptionSpot-Label conjugated to Atto488Size50 µLPrice $ 395
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    Codeeba594-10DescriptionSpot-Label conjugated to Atto594Size10 µLPrice $ 100
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    Codeeba594-50DescriptionSpot-Label conjugated to Atto594Size50 µLPrice $ 395
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    Vimentin-Label

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    CodeDescriptionSizePriceQty
    Codevba488-10DescriptionVimentin-Label conjugated to Atto488Size10 µLPrice $ 100
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    Codevba488-100DescriptionVimentin-Label conjugated to Atto488Size100 µLPrice $ 365
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    Why size matters

    Nano-Boosters & Nano-Labels (also termed fluorophore-conjugated Nanobodies) are much smaller compared to conventional primary plus secondary IgG antibodies (IgG complex). Due to their small size, VHHs are probes for effective labeling with minimal fluorophore displacement for super-resolution microscopy.

    Benefits

    • Higher image resolution and higher labelling density
    • Considerably higher tissue penetration rates
    • Superior accessibility and labelling of epitopes in crowded cellular/organelle environments
    • Less than 2 nm epitope-label displacement minimizes linkage error
    • Stabilization, enhancement and reactivation of fluorescent signals
    • Monovalent VHHs do not cluster their epitopes
    • Consistent and reliable performance due to recombinant production and monoclonality
    • Validated antibodies: structure and function characterized

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    Only for research applications, not for diagnostic or therapeutic use!